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Both optical and chemical (SERS mapping) imaging were used as mechanisms for bacterial detection and quantification. Our assay achieved sensitive and reliable detection of as low as 10 2 CFU mL −1 for Salmonella with great capability for quantification. The total analytical time for optical detection is 1.25 hours and for SERS imaging is 3 hours.

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Afterward, the optimized SERS arrays were replicated on the tips of optical fibers to evaluate their SERS sensing capability. Finally, to investigate a potential in vivo diagnostic application, SERS signals were collected and analyzed from live Escherichia coli (E. coli) cells using both SERS arrays on planar substrates and fiber-optic SERS probes.

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